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Fig. 4 sim1, but not olig2, is expressed in mature DA neurons. A-L: Whole mount in situ hybridization (red) of either Sim1 (A-F) or Olig2 (G-L) followed by anti-TH immunohistochemistry (blue). M-O: Transgenic embryos expressing GFP (green) under the control of the olig2 promoter (Olig2::gfp) stained with anti-TH antibody (blue, pseudo color). A-C, G-I: Dorsal view; D-F, J-O: lateral view. hpf, hours post fertilization; NPO, neurosecretory preoptic area; PT, posterior tuberculum; Tel, telencephalon, Tha, thalamus. Scale bar: 22.5 μm.
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