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Fig. S4 Gata4 is not required for KV development or midline development. (A-C) The expression of charon was not changed in gata4 morphants (B) or in embryos injected with gata4 RNA (C), when compared with controls (A) at the 12-somite stage. Shown are dorsal views of the tail bud region. (D-F) KV cilia development was not affected in gata4 morphants (E) or in embryos injected with gata4 RNA (F), when compared with controls (D) at the 12-somite stage, as visualized by anti-acetylated tubulin antibody staining; this is also summarized by measurement of cilia length (J) and cilia numbers (K). (G-I) Midline structure was not affected in gata4 morphants (H) or in embryos injected with gata4 RNA (I), as revealed by staining for the midline mesendodermal markers gsc and ntl. Shown are dorsal views of tailbud-staged embryos with anterior to the top. Data shown in J and K are means±s.d. Ten to 22 embryos were scored for each experimental group.