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Fig. 3 Distinct bilateral phenotypes in cas, cha, and lft1 morphants. A: Standard analysis of lateral plate mesoderm (LPM) spaw expression categorized as left-side expression (Red), bilateral expression (green), absent expression (blue), and reversed expression (yellow). B-D: Bilateral spaw expression in LPM is categorized as (B) the left side boundary more anterior than that of the right side (left side leading); (C) both sides with equal anterior boundaries (equivalent); (D) right side boundary was more anterior than that of the left side (right side leading). Green arrowhead: leading side spaw expression boundary. E: Quantification of cas, cha and lft1 morphants with bilateral phenotype. F-H: lft1 morphants retain LR bias at 9 to 10 somite stage (SS): (F) spaw expression in the left LPM (n = 28); (G) spaw expression in the right LPM (n = 5); (H) spaw was not expressed in WT (n = 86/88) at this stage. Green arrow: spaw expression.