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Fig. 4 Actin is localized basally at the MHBC in wild type embryos. (A–D) Phalloidin stained wild type (A and C) and sly (B and D) embryos that were fixed at 24 hpf. Stained embryos were flat mounted in glycerol and imaged by confocal microscopy at 25X (A and B) and 100X (C and D). Arrows indicate points of basal actin accumulation in wild type embryos. Anterior is to the left in all images. Arrowhead indicates the MHBC in wild type and a bracket indicates the MHBC region in sly mutants. M, midbrain; H, hindbrain.
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Reprinted from Mechanisms of Development, 125(11-12), Gutzman, J.H., Graeden, E.G., Lowery, L.A., Holley, H.S., and Sive, H., Formation of the zebrafish midbrain-hindbrain boundary constriction requires laminin-dependent basal constriction, 974-983, Copyright (2008) with permission from Elsevier. Full text @ Mech. Dev.