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Fig. S4 Morpholino induced elys cDNA truncation. (A) Ethidium bromide stained agarose gel showing 1 kb truncation of an elys cDNA fragment amplified from 1 dpf and 2 dpf wild type embryos that had been injected with the elys exon 30 splice junction morpholino. This morpholino targets genomic sequence at the intron 29/exon 30 splice acceptor. Successful targeting induces deletion of exon 30, as revealed by sequence analysis (B) of the 0.6 kb fragment.
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