Fig. 3 elys expression in developing zebrafish.
Images (A–H) are whole mount RNA in situ hybridizations. (A) Maternal elys expression at 3 hpf. (B) Strong elys expression is evident in the midbrain (black arrow) and eye (white arrow) at 24 hpf. (C–F) Expression at 48 hpf in the retina (C,E), growth plate of the optic tectum (F), and digestive organs [(D): pancreas (arrow), anterior intestine (white arrowhead), posterior intestine (black arrowheads), liver (*)]. (G–H) Weak elys expression in the 5 dpf anterior (G) and posterior (H) intestine. (I) Graph showing elys expression in whole embryos (4 hpf–120 hpf) as determined via real-time quantitative PCR.