Fig. 5

Fig. 5 Protein product localization in mutant retinae. At 24 hpf, N-cadherin polypeptide is present in wild-type (A), glo^m117 (B) but not in pac^tm101b (C) retinae. This situation persists at later stages of development as shown for the wild-type (D), glo^m117 mutants (E), and pac^tm101b mutants (F). Residual staining present in (C) and (F) is most likely due to background fluorescence. As reported previously in wild-type animals, N-cadherin is expressed in plexiform layers (arrows in D and G) and in the optic nerve (arrowheads D in G). The glo^m117 mutant polypeptide appears to retain this distribution. It is present both in the optic nerve (arrowheads in E and H) and in ectopically localized plexiform patches (arrows in E and H). Western blotting indicates that N-cadherin polypeptide is absent in pac^{tm101b/tm101b} mutant embryos (I) but it persists in glo^m117/m117 animals (J) at 3 dpf. (D, E, G, H) Retinae at 50 hpf. Retina in (F) is at 72 hpf. “le” indicates lens. Arrowheads in (A–C) indicate the midline. In (A–H) dorsal is up.