Fig. 2

Fig. 2 Knockdown phenotype of N-cadherin. Anti-N-cadherin morpholino-modified antisense oligonucleotides reproduce the glass onion phenotype. Gross morphology of the wild type (A), glo^m117 (B), and anti-N-cadherin morpholino-treated animals (C) at 36 hpf. Eye pigmentation of the wild type (D), glo^m117 (E), and morpholino-treated animals (F) at 48 hpf. Tail and somites of the wild type (G), glo^m117 (H), and morpholino-treated animals (I) at 30 hpf. Somite boundaries are poorly defined in both mutant and morpholino-treated embryos. Analysis of cryosections indicates that retinal neuroepithelium is severely disorganized at 28 hpf in morpholino-treated animals (K, L compare with the wild type in J). In (J–L), centrosomes are stained with anti-γ-tubulin antibody (blue), adherens-junction associated actin foci are visualized with Alexa-488-conjugated phalloidin (green), while M- phase nuclei are identified by anti-phosphohistone antibody staining (red). Embryos in (K) and (L) were treated with anti N-cadherin morpholinos 2 and 3, respectively. In (G-I) anterior is right and dorsal is top. “le” in (J–L) indicates lens. Arrows indicate the midline.