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Fig. S6 Wnt attenuation does not affect the barhl2 diencephalic expression domain. Lateral views (anterior to the left) of wild-type (A), lef1 MO-injected (B) or X8 deletion mutant (C) embryos. Embryos were fixed at 24 hpf and subjected to whole-mount in situ hybridization with antisense RNA probe directed against barhl2. All specimens were then subjected to immunostaining with an anti-tyrosine hydroxylase (TH) antibody to detect DA neurons. Scale bar: 50 μm.
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