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Fig. S4 The effect of cell division inhibitors on embryonic development. (A-D) Control embryos were incubated (A,C) in 4% DMSO or (B,D) in a solution containing the cell division inhibitors aphidicolin (APH, 1 μg/ml) and 5-hydroxyurea (5-HU, 50 mM) dissolved in 4% DMSO. Treatment was initiated at 6 hpf, and embryos were cultured overnight until 24 hpf. Lateral views of WT embryos immunostained for phospho-histone H3 (A,B) and of live embryos (C,D) demonstrating that embryogenesis proceeds relatively normally in the presence of cell division inhibitors. Scale bar: 50 μm.
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