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Fig. 8 Canonical Wnt pathway components restrict DA cell number. (A-E) Lateral views of zebrafish embryos that were injected with vehicle solution (WT, n=71; A), wnt8b morpholino (MO) (n=20; B), fz8a MO (n=11; C) or lef1 MO (n=18; D) and of the lef1-deficient X8 deletion mutant (n=18; E). Embryos were fixed at 24 hpf and subjected to in situ hybridization with a dat probe. The result of the double knockdown of wnt8b and fz8a (n=44) is presented in F. (F) Bar chart summarizing average cell counts of DA group 2 neurons in the genetic backgrounds shown in A-E. (G,H) Lateral views (anterior to the left) of embryos that were injected with vehicle solution (G) or wnt8b MO (H). Embryos were fixed at 24 hpf and subjected to whole-mount in situ hybridization with an RNA probe directed against barhl2. Scale bar: 50 μm.