ZFIN Image: Del Bene et al., 2008, Fig. S7

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Figure Caption

Fig. S7 moks³⁰⁹ Mutants Have Normal Apical-Basal Marker Distribution
(A-D) 28 hpf retina sections stained with an antibody recognizing the basal lamina marker Laminin (green in [A, B]) and the apically distributed markers aPKCegreen in [C, D]) and the cell junction component ZO-1 (red in [C, D]). No obvious difference is detected between mutant (B, D) and wildtype (A, C) retinas.
(E,F) 28 hpf retina section stained with phalloidin (red) to detect a-actin and expressing Pard3-GFP (green). The majority of the Pard3-GFP protein is localized at the apical surface where it overlaps with the actin rich adherens-junctions. Nuclei of cells expressing the Pard3-GFP construct are marked by white stars. Nuclei counterstained with DAPI in blue. Scale bars 25 μm.

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Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell