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Fig. 2 Simultaneous expression of Cx55.5 and its carboxy-terminal domain. A) Nucleotide sequence of the 5′ end of the carboxy-terminal domain, with in-frame ATG codons at nucleotide positions 634, 643 and 1201 shown in bold. B) Schematic representation of EGFP-fusion protein constructs of full length Cx55.5 (WT, I; nt1 to nt1497), carboxy terminal domain of Cx55.5 (II; CT-629; nt629 to nt1497) and 3′ half of carboxy-terminal domain (III; CT-1196; nt1196 to nt1497). C) Western blot analysis of transiently transfected N2A cells with EGFP fusion constructs: (lane I) full length Cx55.5, (lane II) full length carboxy-terminal domain (CT-629), (lane III) 3′ half of the carboxy-terminal domain (CT-1196). Note that in lane I (full length Cx55.5 construct), besides 83 kDa and 38 kDa fusion protein bands, a few putative N-terminally truncated Cx55.5 protein bands are also visible.