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Fig. 3 olig3 knockdown affects differentiation of lateral neural plate cells. (a–f) isl1/2-expressing interneurons (a and b; black arrowheads in a) (18, 19) are absent in 20-somite (b and d) and 24-hpf (f) embryos injected with olig3MO. Conversely, RB cells (RB) are increased in number in olig3 morphants (b, d, and f) (morphant isl1-phenotype > 90%, n = 40; morphant isl2-phenotype > 90%, n = 30). mn, motoneurons. (g and h) lim1 is expressed at 24 hpf in D3 interneurons of the dorsal spinal cord (g). D3 interneurons are slightly affected in embryos injected with olig3MO (h) (morphant phenotype > 90%, n = 15). (i and j) The glial fibrillar acidic protein gene (gfap) is expressed at 3 days postfertilization in astrocytes of the dorsal spinal cord (i). gfap expression is absent in embryos injected with olig3MO (j) (morphant phenotype > 90%, n = 20). Embryos are in transverse sections (a and b) or in lateral view with anterior to the left (c–j).