Fig. 2

Fig. 2 appl Developmental Expression Profiles
(A) appl1 and 2 mRNA patterns during early embryogenesis, revealed by antisense probes. Lateral views. %, % epiboly; ss, somites. Both genes are ubiquitously expressed.
(B) Simultaneous ISH with an appl1 sense probe. No unspecific background staining was observed.
(C) appl1 and 2 mRNA expression at 24 hr of zebrafish development. Left panels, heads in lateral view; mid panels, heads from top; right panels, tails in lateral view. High expression of appl1 is observed in telencephalon (arrows), olfactory pits (asterisks), and pronephros (arrowheads).
(D) appl1 and 2 mRNA expression at 48 hr of zebrafish development. Left panels, dorsal views; right panels, ventral views on the zebrafish trunk. appl2 mRNA is enriched in the olfactory placode (asterisks).
(E) Sections through appl1 (panels I–III) or appl2 (panels IV and V) whole-mount ISH of 30 hr zebrafish. Positions of sections are indicated in the scheme. Empty arrows, high expression of appl1 and appl2 in specific zones of the hindbrain (I and IV) and neural tube (II, III, and V). Arrowheads, appl1 in the pronephric duct.
(A–E) appl1 and 2 whole-mount ISH (in violet) on zebrafish embryos of indicated developmental stages. The scale bars in (A) and (B) represent 250 μm; in (C) and (D), 200 μm; and in (E), 50 μm.
(F) Antibodies to Appl1 and Appl2 recognize single bands of 80 and 75 kD in immunoblots on ZF4 extract.
(G) Western blot analysis of staged zebrafish embryos using anti-Appl antibodies. Anti-γ-tubulin was used as loading control.