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Fig. 2 Testis immunohistochemistry of mlh -/- zebrafish. a, b TUNEL staining of mlh1 +/+ and mlh1 -/- fish, respectively. Large numbers of apoptotic cells can be seen in mutant testis (brown). Wild-type testis exhibits a low incidence of apoptosis. c, d Histone H3 staining of cells in metaphase from mlh1 +/+ and mlh1 -/- fish, respectively. Note the higher incidence of stained cells in mutant testis indicating a higher number of mitotic and meiotic cells in metaphase. Bars 25 &mum (a, b), 50 μm (c, d)
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