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Fig. S2 In situ hybridization with pac1, isotocin (it) and fezl RNA probes. (A,B) High resolution micrographs (lateral view) of embryos which were subjected to in situ hybridization with RNA probes directed against pac1 followed by anti-TH immunohistochemistry. (C) Double in situ hybridization with RNA probes directed against isotocin (it) and fezl (ventral view). (D,E) Wild type (WT; D) and tofm808 (E) mutant with antisense fezl probe followed by anti-TH immunohistochemistry.
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