Fig. 6

Fig. 6 Cdx4 functions in the endoderm to localize the pancreas. (A,C,E) Cell transplantation strategy. Donor and host zebrafish embryos were injected at the one-cell stage with the indicated reagents. At the sphere stage, donor cells were transplanted to host blastoderm margin. (B) cdx4 knockdown in endoderm is sufficient to shift the pancreas posteriorly. (D) Wild-type endoderm rescues the pancreas location in a cdx4-knockdown host. (F) Cdx4 overexpression in endoderm results in insulin expression anterior to the normal pancreatic domain. Embryos at 24hpf, dorsal view. Magnification: 100x.