Fig. S3

Fig. S3 C-myb, scl, ikaros, and runx1 Expression in the Developing Hematopoietic Tissues (A-F,I) lateral views, rostral left; (G,H) ventral views, rostral up; (A) ventral tail at 2 dpf; the area boxed in (a) is displayed enlarged and at a shallower focus in (b), to show that c-myb+ cells are comprised between the caudal artery (ca) and the somite muscle (s) ventral limit (arrows), except for a few in the more ventral CV plexus (cvp) lumen. (B,C) expression in thymus of ikaros at 3 dpf (B) and c-myb at 4 dpf (C); note the single positive cells outside of the thymus anlage, caudalwards (arrowheads); oc, otic capsule. (D-F) From 3 to 7 dpf, scl hematopoietic expression progressively moves from the tail (arrowhead) to the kidney (red arrow); scl+ circulating cells accumulate in the heart (green arrowhead); (G,H) Ikaros (G) and runx1 (H) expression in the 5 dpf kidney, around the pronephric glomerulus (g); (I) Ikaros expression in the 6 dpf tail. Bars, 50 μm