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Fig. 1 isl1, fgf10 and meis3 expression adjacent to the pancreatic ventral bud. Embryos analyzed by whole-mount in situ hybridization for the expression of isl1, fgf10 and meis3. Images are ventral views of the trunk embryo, with the anterior oriented to left and the left side of the embryo to the top. (A) isl1 expression from 24 to 36 hpf. (B) fgf10 expression at 28 and 36 hpf. (C) meis3 expression from 24 to 36 hpf. Dotted yellow lines highlight the bilateral expression domain. (D) Double-labeled whole-mount in situ hybridization showing the expression of isl1, fgf10 and meis3 compared with ptf1a. Yellow arrows indicate expression of isl1, meis3 and fgf10 in a tissue adjacent to the ventral pancreatic bud. DB, dorsal pancreatic bud; FB, pectoral fin bud; VB, ventral pancreatic bud. Magnification, 200x.