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Fig. 4 zFmi1a functions in prechordal plate migration in concert with Slb/Wnt11 and in convergence of the neural plate. Whole-mount in situ hybridization markers are as indicated on the plates, (A, B, G–O) bud stage, (C–E) 10-somite stage, (P–R) and 12-somite stage. (A and B) Lateral views, dorsal is to right. (C–E) Dorsal views, anterior is to top. (C) pax2.1 in optic stalk is marked by an arrow. K is krox20 expression in panel E. (F) Titration of F1a-GPS1 morpholino to generate 50% wild-type and 50% mis-spliced pre-mRNA at 3 μg/ml. CoMo is F1a-GPS1 morpholino but with four nucleotide changes. Molecular weight markers (bp) are indicated. (G–O) Embryos were derived from crossing of Slb heterozygotes and injected with either CoMo (same as in F) at 3 μg/ml (G–J) or F1a-GPS at 3 μg/ml (K–O). (G, I, K–M) Lateral views, dorsal is to right. (H, J, N, and O) Dorsal views, anterior is to top. (M) Arrowhead indicates loss of dlx-3 expression in dorso-lateral neural plate and black arrow indicates broader and more intense expression of dlx3 within the ventro-lateral neural plate. (P–R) Wild-type embryos injected with morpholinos as indicated. Dorsal view of head, anterior is to top. (P) Arrow indicates pax2.1 expression within optic stalk, (Q) F1a-GPS1 at 3 μg/ml. (R) Double morphants, F1a-GPS1 (3 μg/ml) plus Wnt11 morpholino.
Reprinted from Developmental Biology, 282(2), Formstone, C.J., and Mason, I., Combinatorial activity of Flamingo proteins directs convergence and extension within the early zebrafish embryo via the planar cell polarity pathway, 320-335, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.