ZFIN Image: Machingo et al., 2006, Fig. 2

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Figure Caption

Fig. 2 β4GalT1 is expressed throughout the first 24 h of embryonic development. (A) Semi-quantitative RT-PCR for β4GalT1 in staged RNA libraries; oocyte, 50% epiboly, 12-somite and Prim-16. Expression is first detected in the oocyte and remains relatively constant in all stages assayed. Error bars = ±SEM. (B, C) In situ hybridizations for β4GalT1. (B) Two-somite stage, lateral and dorsal views, display β4GalT1 expression throughout the embryo. The increased signal in the midline (arrow) is most likely due to increased tissue mass. (C) 26-somite stage embryos; lateral, dorsal at the midbrain level, and nasal views are shown, demonstrating tissue-specific increases in expression. β4GalT1 expression in the lateral mesoderm ends abruptly at the midbrain level (asterisks) and shows high expression in the developing retina (arrowhead) and nasal placode (arrow).

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 297(2), Machingo, Q.J., Fritz, A., and Shur, B.D., A beta1,4-galactosyltransferase is required for convergent extension movements in zebrafish, 471-482, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.