Fig. 9

Fig. 9  Histological comparison of adult wild-type mosaic, lens-ablated, and bal^a69 mosaic eyes. (A–D) Iridocorneal angle analysis in (A) wild-type mosaics showed normal morphology including annular ligament and ciliary canal. In (B) lens-ablated fish, cell differentiation in the angle appeared normal, although the ciliary canal was often narrow. Angle differentiation in (C, D) bal^a69 mutant mosaics was often abnormal including defects in the annular ligament (arrows), iris, and ciliary margin. In the posterior region, the iridophore layer (arrowheads) appeared normal in (E) wild-type mosaics and (F) lens-ablated fish but was often thick in (G) bal^a69 mutant mosaics. Whereas the retinal ganglion cell (RGC) layer was dysmorphic in both lens-ablated and bal^a69 mutant mosaics with lens degeneration, focal dysplasias within the RGC layer were also observed in (I) bal^a69 mutant mosaics with normal lenses. No dysplasias or other phenotypes were noted in (H) wild-type mosaics.