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Fig. 6 Rescue of cal with human ATP7A and transplantation experiments
The calamity mutant phenotype (A) is rescued by injection with synthetic RNA encoding the human Menkes ATPase (B). Although cal embryos display a complete lack of melanin pigment at 48 hpf (C), transplantation of rhodamine-dextran-injected wild-type cells into cal embryos (D) yields embryos mosaic for pigmented melanophores (arrows). In all cases, the pigmented cells shown here in the retinal epithelium (E) correspond to the rhodamine-labeled donor cells as detected by fluorescence for CY3 in this same epithelium (F).
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Reprinted from Cell Metabolism, 4(2), Mendelsohn, B.A., Yin, C., Johnson, S.L., Wilm, T.P., Solnica-Krezel, L., and Gitlin, J.D., Atp7a determines a hierarchy of copper metabolism essential for notochord development, 155-162, Copyright (2006) with permission from Elsevier. Full text @ Cell Metab.