Fig. 5

Fig. 5 Differential expression of CRMP mRNAs in the hindbrain and trunk region at 24 hpf. Probes used are indicated in the photomicrographs. (A) In a top view of an embryo labeled with a CRMP-4 probe (rostral is left), serially repeated clusters of neurons (arrowheads) can be observed in the hindbrain (hb). Additional signal is present in the nucleus of the medial longitudinal fascicle (nmlf), the trigeminal ganglion (tg), the acoustic/anterior lateral line ganglion (ac/allg) and the posterior lateral line ganglion (pllg). The otic vesicle (ov) is indicated for orientation. (B,C) In cross-sections of spinal cord (dorsal is up) labeled with CRMP-3 (B) or CRMP-4 (C) cRNA probes, a signal in the lateral domain, containing differentiated neurons, is obvious. Large Rohon-Beard neurons (RhB) strongly express CRMP-4 mRNA. (D–I) Side views of mid-trunk regions are shown (rostral is left, dorsal is up). Pairs of horizontal bars delineate the dorso-ventral extent of the spinal cord. CRMP-2 and -4 mRNAs are prominently expressed in Rohon-Beard neurons, some of which are indicated by arrowheads in E,G. Arrowheads in F indicate CRMP-3 mRNA positive spinal interneurons. Expression of CRMP-5b mRNA is weak in the spinal cord (arrowheads in I) and CRMP-1 mRNA was not detected in the spinal cord. Scale bar in A, 100 μm; scale bar in C, 25 μm for B, C; scale bar in I, 100 μm for D–I.