Fig. 1 Expression of lhx2 and lhx9 mRNA in the zebrafish embryo. (A) Expression of lhx2 in the presumptive forebrain at the early somitogenesis stage (11 hpf) (A), at 24 hpf (B) and at 32 hpf (C, D). (E–H) Expression of lhx9 in the zebrafish brain at the same stages as A–D. (A–C, E–G) Dorsal views, anterior up; (D, H) lateral views, anterior left, dorsal up. t, telencephalon; ov, optic vesicles; white arrowhead (C) and e (D), epiphysis; black arrowheads, hindbrain segments; asterisk, mid–hindbrain boundary. (I) Double labeling of lhx9 expression (purple) and primary neurons (brown) by in situ hybridization and α-Tb immunostaining in a 30-hpf embryo. poc, postoptic commissure; TPOC, the tract of the postoptic commissure; arrowheads, complementally localization of lhx9-expressing cells and the reticulospinal neurons in the hindbrain. Scale bar, 100 μm.
Reprinted from Developmental Biology, 287(2), Ando, H., Kobayashi, M., Tsubokawa, T., Uyemura, K., Furuta, T., and Okamoto, H., Lhx2 mediates the activity of Six3 in zebrafish forebrain growth, 456-468, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.