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Fig. S4 Aida does not affect binding affinities between Axin and MEKK1-CT, MEKK4-CT or disheveled. (A) Cells were transfected with HA-MEKK1-CT and FLAG-Axin together with increasing amount of Myc-Aida (0, 1, 3 μg). Immunoprecipitation and western blotting using different antibodies were carried out as above described. (B and C) Similar experiments to that in A were performed except for the use of HA-MEKK4-CT (B) and HA-Dvl (C).
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Reprinted from Developmental Cell, 13(2), Rui, Y., Xu, Z., Xiong, B., Cao, Y., Lin, S., Zhang, M., Chan, S.C., Luo, W., Han, Y., Lu, Z., Ye, Z., Zhou, H.M., Han, J., Meng, A., and Lin, S.C., A beta-Catenin-Independent Dorsalization Pathway Activated by Axin/JNK Signaling and Antagonized by Aida, 268-282, Copyright (2007) with permission from Elsevier. Full text @ Dev. Cell