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Fig. 12 Foxe3 protein expression is reduced in pitx3 morphants. Immunoblot analysis of protein expression in the pitx3 morphants was used to further assess possible genetic interactions between pitx3 and foxe3. The pitx3 morphants (M) lacked Pitx3 protein at 3 and 4 dpf, while wild-type levels of Pitx3 were detected in both the mismatch control morpholino-injected (mmC) and uninjected (uiC) controls (panel A; arrow). At 5 dpf, Pitx3 protein was detected in the pitx3 morphants and the controls. Panel B demonstrates that Foxe3 protein is not detected in the pitx3 morphants (M) at 3 and 4 dpf, but is present in both controls (mmC and uiC). In 5 dpf morphants, Pitx3 and Foxe3 are present at nearly normal levels (panels A and B, respectively). Actin detection within the extracts demonstrates equivalent protein loading of the lanes (panel C).
Reprinted from Mechanisms of Development, 123(10), Shi, X., Luo, Y., Howley, S., Dzialo, A., Foley, S., Hyde, D.R., and Vihtelic, T.S., Zebrafish foxe3: Roles in ocular lens morphogenesis through interaction with pitx3, 761-782, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.