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Fig. 5 Immunoblot analysis of morpholino-mediated Foxe3 knockdown. Protein was extracted from foxe3 morphants and controls for immunodetection using anti-Foxe3, anti-Pitx3 or anti-β-actin serum. At 3 and 4 dpf, Foxe3 protein is not detected in the morphants (M), while wild-type Foxe3 levels are expressed in the mismatch control morpholino-injected (mmC) and uninjected (uiC) controls. At 5 dpf, nearly equivalent amounts of Foxe3 are detected in the foxe3 morphant and both controls. In comparison, the foxe3 morphants express wild-type levels of both Pitx3 and β-actin at all three ages.
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Reprinted from Mechanisms of Development, 123(10), Shi, X., Luo, Y., Howley, S., Dzialo, A., Foley, S., Hyde, D.R., and Vihtelic, T.S., Zebrafish foxe3: Roles in ocular lens morphogenesis through interaction with pitx3, 761-782, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.