Fig. 5

Fig. 5 Comparison of embryonic expression of dermacan and versican by whole-mount in situ hybridization. Dorsal views with anterior to the top (A–D,F,H,I,K). Lateral views with anterior to the top (O–R,T,U) and anterior to the left (E,J,M). (A–G) dermacan expression was first detected in the sclerotome and cephalic paraxial mesoderm (open arrow) at 16 hpf (B) and becomes more evident as embryos develop (C, 19 hpf). The expression of dermacan was observed in the cluster of cells between the midbrain and the yolk (arrowhead) at 24 hpf (D), cranial region (arrow) at 31 hpf (E), and otic vesicle and pectoral fin buds at 48 hpf (F). (G) A cross-section of F. (H–N) The expression of versican was observed in adaxial cell and lateral plate mesoderm before dermacan expression (H, 14 hpf, I, 16 hpf), lens primordium and paraxial mesoderm at 19 hpf (J), heart at 24 hpf (K), and otic vesicle at 48 hpf (M). (L) A cross-section in panel J shows the signal in the lens at 24 hpf. (N) A cross-section of M. (O–R) The expression of dermacan in the sclerotome and tail fin bud. dermacan expression was detected in the sclerotome at 24 hpf (O), 27 hpf (P), and 31 hpf (Q), but was not detected at 41 hpf (R). A mesenchymal signal in the tail fin bud was strongly detected at 41 hpf (R). (S) A cross-section of E. versican was detected in tail fin bud at 24 hpf (T) and 27 hpf (U), but was undetectable at 31 hpf (data not shown). (a) Diagram showing the embryo at 23 hpf. (b–d) Cross-sections of embryo in panel a, indicating the signal in the ventral portion of the somites and around the notochord. Abbreviations: ad, adaxial cell; fb, tail fin bud; h, heart; hb, hindbrain; lm, lateral plate mesoderm; lp, lens primordium; n, notochord; ov, otic vesicle; pf, pectoral fin; pm, paraxial mesoderm; sc, sclerotome.