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Fig. 5 Injection of phox2b morpholinos generates partial phenocopies of the sox10 enteric neuron phenotype. Lateral views of posterior hindgut of 5 dpf wild-type embryos injected with 2 ng dose of phox2b.a (C, D) morpholino to show severe reduction in numbers of Hu-positive enteric neurons (arrows) compared with uninjected controls (A, B). DIC images of same embryos confirm their normal gross morphology (A, C). hg, hindgut; a, anus.
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Reprinted from Mechanisms of Development, 122(5), Elworthy, S., Pinto, J.P., Pettifer, A., Cancela, M.L., and Kelsh, R.N., Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent, 659-669, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.