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Fig. 4 Slow muscle migration defects in cxcr4a and sdf1a morphants. Confocal images of embryos stained for slow myosin using F59 antibody. Dorsal (A-C;G-I) and lateral (D-F) views of embryonic trunk between the fourth and tenth somites. (A-C) Adaxial cells in cxcr4a and sdf1a morphants are identical to that in controls, 19 h. (D-I) Embryos at 25 h. (D-F) Z-stacked images of ten frames. (G-I) Z-stacked images of two frames. (D) Distinct and properly aligned slow fibers are seen in control embryo. (E,F) Gaps are seen in myotomes of representative cxcr4a and sdf1a morphant, indicated by white arrows. (G) Control. (H,I) Loss of fiber at the superficial layer and misrouted slow muscle, indicated by white arrows in representative cxcr4a and sdf1a morphant respectively. Other misrouted slow fibers in morphants are in different planes (data not shown).