Fig. S1

Fig. S1 Comparison of the notochord distortion obtained in zebrafish using three distinct methods of lysyl oxidase inhibition at 24 hpf. Wild-type embryos were incubated in 10 μM neocuproine (A), 10 mM ®-aminopropionitrile supplemented with 10 μM CuCl2 (B), 100 mM ®-aminopropionitrile supplemented with 10 μM CuCl2 (C), or injected with 6 ng each of loxl1 and loxl5b start (ATG) morpholino (D). 10 μM neocuproine (A) causes greater notochord distortion than 10 mM ®-aminopropionitrile (B), but an identical phenotype is obtained with 100 mM ®-aminopropionitrile (A vs. C). Melanin pigmentation has not yet developed in the embryos pictured, but is not affected by ®-aminopropionitrile treatment or by the morpholino injection.