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Fig. 1 Igf1rb is required to establish normal primordial germ cell (PGC) numbers at the prim-5 stage. Immunohistochemical identification of PGCs (Vasa-positive cells, arrows) in prim-5 stage zebrafish embryos, after ablation of Type-1 IGF receptors (igf1ra, igf1rb): (A) control (non-targeting) morpholinos; (B) igf1ra-MO; (C) igf1rb-MO; (D) igf1ra-MO + igf1rb-MO. Magnification, 200x. Mean PGC numbers of treatment groups are summarized in Table 1.
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Reprinted from Developmental Biology, 305(1), Schlueter, P.J., Sang, X., Duan, C., and Wood, A.W., Insulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survival, 377-387, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.