ZFIN Image: Schäfer et al., 2005, Fig. 3

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Figure Caption

Fig. 3

Differences in the expression patterns of nkx2.2a and nkx2.2b. nkx2.2a (A) and nkx2.2b (B) expression in embryos at the 18s stage. (C-F) Higher magnification of rostral (C,D) and caudal (E,F) head regions showing nkx2.2a (C,E) and nkx2.2b (E,F) expression in the ventral brain. Note differences in expression in the mid-diencephalic boundary (MDB), the mid-hindbrain boundary (MHB) and in the pancreatic progenitor region (pc). (G-J) Transversal sections showing expression of nkx2.2a (G,I) and nkx2.2b (H,J) in the ventral CNS at hindbrain (G,H) and trunk (I,J) levels (positions of sections indicated in A,B). (K,L) Gradual reduction of nkx2.2a (K), but maintenance of strong nkx2.2b expression (L) in the posterior trunk. (M-S) Transversal sections through the spinal cord of 18s wild-type embryos showing expression of foxa2 (M), nkx2.2b (blue) and shh (red) (N), tal2 (O), olig2 (red) and nkx2.2b (blue) (P), isl1 (Q), isl1 and nkx2.2b (R) at mid-trunk level, and pax6 at posterior trunk level (S). LFP, lateral floor plate; MDB, mid-diencephalic boundary; MFP, medial floor plate; MHB, midbrain-hindbrain boundary; mn, motoneurons; nc, notochord; pc, pancreatic progenitor cells; sn, sensory neurons. (A-F) lateral views and (K,L) dorsal views, anterior to the left. Scale bar in (B) represents 200 µm (for A,B), in (D) 100 µm (for C,D), in (F) 50 µm (for E,F), in (L) 30 µm (for K,L), and in (S) 20 µm (for G,I,H,J,M-S).

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Mechanisms of Development, 122(1), Schäfer, M., Kinzel, D., Neuner, C., Schartl, M., Volff, J.N., and Winkler, C., Hedgehog and retinoid signalling confines nkx2.2b expression to the lateral floor plate of the zebrafish trunk, 43-56, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.