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Fig. 6 Elevated cell death in disarrayed retinas. Acridine orange (AO) was used to label dying cells in the living zebrafish embryo. The average number of positive cells per retina in wild-type (grey bars) and disarrayed embryos (black bars) is presented. Inset shows chromatin fluorescence (green) in wild-type (left) and disarrayed (right) embryos following acridine orange treatment at 48 hpf. Note that there are significantly more cells labeled in the disarrayed mutant retina and forebrain when compared to wild-type. ** p ≤ 0.01 (Student's t-test). n = total number of embryos counted.
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Full text @ BMC Dev. Biol.