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Fig. 8 Somitogenic and cyclic expression defects from her7 mRNA over-expression. The expression of myoD in the presomitic mesoderm and trunk somites of embryos at 14 hpf (10 som, A, B) and cycling genes in the presomitic mesoderm at 10 hpf (1 som, F–I) are shown in dorsal view after flat mounting with anterior up. The myotome boundaries of the trunk marked by titin expression are shown in 26 hpf embryos in lateral view, anterior to the left and dorsal up (D, E). Scale bar in panel D is 250 µm. Arrows and arrowheads indicate localized defects and brackets indicate the extent of larger regions of abnormalities. Expression of myoD after injection of lacZ (A) or her7 mRNA (B). Segmentation defects of the trunk coincide with the region in which the Myc-tagged Her7 protein can be detected (C). Segmentation of the trunk after injection with lacZ mRNA (D) or her7 mRNA (E). Expression of delC (F, G) and her1 (H, I) after the injection of gfp (F, H) or Myc–her7 mRNA (G, I).
Reprinted from Developmental Biology, 304(2), Shankaran, S.S., Sieger, D., Schroter, C., Czepe, C., Pauly, M.C., Laplante, M.A., Becker, T.S., Oates, A.C., and Gajewski, M., Completing the set of h/E(spl) cyclic genes in zebrafish: her12 and her15 reveal novel modes of expression and contribute to the segmentation clock, 615-632, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.