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Fig. 4 Effects of amotl2 expression knockdown on embryonic cell movements. (A) Injection of amotl2 morpholino led to slower epiboly and impaired CE movement. Single-cell embryos were injected with amotl2MO1 (MO1), amotl2MO2 (MO2) or control morpholino (cMO1) at the indicated doses. The same embryo was pictured every 2 hours and presented here. Embryos are shown as lateral views with animal pole to the top and dorsal to the right. Arrows denote the anterior tip of the hypoblast, and arrowheads point to the tail region. (B) Injection of amotl2 morpholino affected convergent extension. Injected embryos at the two-somite stage were fixed and simultaneously hybridized to hgg1 (red), dlx3 (blue) and ntl (blue) probes. Each embryo was placed in different orientation: in the top panel, anterodorsal views show relative positions of the hgg1 and dlx3 domains; in the middle panel, dorsal views show the ntl domain; and in the bottom panel, lateral views with dorsal to the right show the length of the ntl domain and the embryonic anteroposterior axis. (C) Effect of amotl2 morpholino injection was alleviated by overexpression of modified amotl2 (amotl2m) mRNA. Groups of live embryos were pictured at the indicated stages.