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Fig. S3 Midline structures and patterning are not impaired by Arhgef11 loss-of-function. In situ hybridization using olig2 probe on cryosections of WT control (A) and MOAUG-injected (B) embryos indicates normal patterning of the neural tube (outlined by white dotted lines). Notochord also appears normal (outlined by black dotted lines). In situ hybridization using probe for the Hedgehog target gene, patched1, on WT (C,C2) and morphant embryos (D,D2) also reveals no abnormalities. Probes for olig2 and patched1 were as described by Park et al. (Park et al., 2002) and Concordet et al. (Concordet et al., 1996), respectively.