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Fig. 4 jagged 2 expression in multiciliated cells. (A,B) Whole-mount in situ hybridization showing expression of jagged 2 (arrowheads) at the 18-somite stage in cells of the intermediate mesoderm (A) and in individual cells of the pronephros at 42 hpf (B). (C) Cross section of the pronephros double stained for acetylated tubulin (HRP:DAB immunohistochemistry) and jagged 2 (in situ hybridization) shows that isolated jagged 2-positive cells possess apical cilia bundles (arrow). Dashed line outlines the cross section of the pronephric tubule. (D) Double in situ hybridization with the MCC marker shippo1 (red) and jagged 2 (blue) demonstrates jagged 2/shippo1 co-expression in isolated single cells. (E-G) Confocal optical section of the pronephros in situ hybridized for shippo1 mRNA (E; green) and jagged 2 (F; red) confirm jagged 2 expression in shippo1-positive MCCs. (G) Merged image. Dotted lines outline the pronephros.