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Fig. 6 Treatment of embryos with the fibroblast growth factor (FGF) signaling blocker su5402 partially phenocopies fgf1 morphants. Labeling of panels is as in Figure 2. Embryos shown here were treated with 10 μM su5402 from 22 hours postfertilization (hpf) to fixation at 32 hpf. A-D: Expression of gata1 and ika is increased and can be found in an enlarged domain in the intermediate cell mass (ICM) in fgf1 morphants (arrows in B,D). E-H:o-Dianisidine staining shows that, in contrast to fgf1 morphants, erythrocyte differentiation is not significantly delayed in su5402-treated embryos. Cells accumulating in the posterior ICM differentiate (arrow in H).