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Fig. S2 Endoderm and dorsal forerunners cells, which ultimately form Kupffer's vesicle, appear normal in morphants. In situ hybridization using sox17 probe on 8.5 hpf uninjected WT (A,A′) and MOAUG-injected (B,B′) embryos demonstrates formation of endoderm and dorsal forerunner cells (arrows) is not disrupted in morphants. Embryo length (marked by arrowheads) is also unaffected. Probe used was as described in Alexander and Stainier (Alexander and Stainier, 1999).
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