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Fig. 7 Ectopic otic induction after fgf8 expression at late gastrulation stages requires both, Foxi1 and Dlx3b. (A-D) Inactivation of Dlx3b or Foxi1 in wild-type embryos by morpholino injection (MO) leads to a reduction of ear size in comparison to wild-type embryos, and combined loss of Dlx3b and Foxi1 results in loss of all indications of otic specification. (E-H) Ear size reduction by depletion of Dlx3b or Foxi1 but not combined loss of Dlx3b and Foxi1, can be partially rescued in transgenic hsp:fgf8 embryos heat shocked at late gastrulation stages. Lateral views of otic vesicles hybridized with starmaker at 24h with anterior to the left and dorsal towards the top. Scale bar: 40 μm.