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Fig. 7 Apoptosis in ris embryonic and adult erythrocytes. (A) Colormetric TUNEL assay of wild-type and ris embryos at 4 dpf, shown in ventral view with head up. 10x magnification. No staining is observed in cardiac chamber (arrowhead) of wild-type embryo, whereas marked apoptosis is observed in red cells pooled in cardiac chamber of ris. (B) Fluorescence confocal TUNEL analysis of kidney and peripheral blood from wild-type and ris adults. The number of erythrocytes is severely reduced in the ris peripheral blood, where a larger fraction is undergoing apoptosis (green cells). The kidney of ris is hyper-cellular, with low level of apoptosis (green).