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Fig. 4 Sptb product is not detected in ris blood cells. (A) Wholemount immunohistochemical detection of Sptb product with anti- BSct polyclonal antisera in wild-type and ris embryos at 23 hpf. (B) Western blot detection of Sptb from whole-blood lysate collected from wild-type, ris/+ and ris/ris adult fish with anti-BSct polyclonal antisera (arrow), and normalized for sample loading with anti-Band3 polyclonal antisera. The zebrafish Sptb protein is >220 kDa in size.
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