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Fig. S1 Cell autonomy of sox10 action in DRG sensory neurons. Fluorescein dextran-labelled wild-type donor cells were transplanted into unlabelled sox10 mutant host embryo, and differentiated sensory neurons identified by anti-Hu immunofluorescence (red) at 3 dpf. Embryo is shown as a projected confocal stack. Transplanted cells (green) contributed to three adjacent DRGs, but whereas Hu+ neurons are found in two of them, the third (*) lacks sensory neurons despite having a large contribution of wild-type cells to glial component. Note that all other fluorescein+ cells lie within the CNS. Among our transplants, two separate individuals showed a result equivalent to that documented here. Thus, wild-type glia are insufficient for survival of DRG sensory neurons.