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Fig. 6 elavl3 (HuC) expression in cervical sympathetic ganglia of wild type and hands off mutants. elavl3 whole-mount in situ hybridizations viewed from dorsal. elavl3-labeled sympathetic ganglia (dashed circle) and enteric ganglia (white arrowhead) are indicated. Expression of elavl3 in control and hands off embryos at 2 dpf (A,B), 3 dpf (C,D) and 4 dpf (E,F). (G) Area of elavl3-expressing cells at 2, 3 and 4 dpf in hands off mutants compared with wild type. (Number of embryos analysed: 13 wild type, six mutant for 2 dpf; 36 wild type, 13 mutant for 3 dpf; nine wild type, seven mutant for 4 dpf). Data are presented as mean ± s.e.m.; n.s. not significantly different; ***P<0.001; asterisks label elavl3 (HuC) staining of epibranchial placodes.