ZFIN Image: Raymond et al., 2006, Fig. 5

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Figure Caption

Fig. 5 Injury-induced retinal progenitors regenerate cone photoreceptors within a week. A) At 4 dpl, proliferating cells (BrdU; magenta) fill the lesioned area (asterisks) in which double cones immunoreactive for zpr1 (green) are missing. Scale bar = 250 μm. B) By 7 dpl, some retinal progenitors that were labeled with BrdU (magenta) at 4 dpl have begun to differentiate into cones and are double-labeled with zpr1 (green). Boxed areas are shown at higher magnification in the insets; double-labeled cells are white. C) By 31 dpl fully differentiated, regenerated cone photoreceptors (zpr1; green) are labeled with BrdU (magenta) injected at 3 dpl (double-labeled white nuclei are indicated by black arrows). Unidentified BrdU⁺nuclei are seen in the inner retina. D) Cocktail of riboprobes to cone opsins (green) identifies BrdU⁺(magenta), regenerated cones at 31 dpl (double-labeled white nuclei are indicated by black arrows). BrdU⁺rod nuclei (magenta) are in the inner part of the outer nuclear layer, ONL. E) Müller glia in a transgenic zebrafish Tg(gfap:GFP)^mi2001are labeled with anti-GFP (green) and co-express apoE (magenta, in situ hybridization) in their cell bodies. F) At 4 dpl, most BrdU⁺proliferating nuclei are in the outer nuclear layer, ONL, but a few apoE⁺Müller glial cells are also BrdU⁺(inset). Radial fibers of Müller glia are labeled with zpr1/anti-GFAP (blue).

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.