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Fig. S1 Localised activation of MAPK activity at the onset of gastrulation. ERK activity is detected with a dorsoventral gradient of activity in the mesendoderm progenitors by anti di-phosphorylated ERK antibody during different stages of development. (A) Dome stage, (E) 30% epiboly, (B,F) shield stage. (C) ERK activity is expanded towards the animal pole in ERK2*-injected embryos and is completely lost in SU5402-treated embryos (G). (D,H) sprouty4 expression at 60% epiboly. (H) Treatment with SU5402 induced the complete loss of sprouty4 expression.
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