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Fig. 5 Alkaline phosphatase labeling of neuromasts. A: Normal pattern of neuromasts in a 2-day-old embryo. L1-L5 and the terminal neuromasts are derived form primI. D1, the first neuromast of the dorsal line, is derived from primII. B: Normal pattern in a 6-day-old larva. Consecutive primI neuromasts are connected by a thin trail of interneuromastic cells. Four additional neuromasts have been added by primII: LII.1-LII.4. The dorsal line comprises three additional neuromasts along the dorsal midline (out of focus). C: The trail of primI interneuromastic cells (INC) is continuous with the primI neuromast L1 but appears pushed away by the derivatives of primII (D1 and LII.1). D,E: The phosphatase activity reveals neuromast anisotropy, with the primI neuromasts being polarized along the antero-posterior axis while the primII neuromasts are polarized in a dorso-ventral direction (double arrows). Scale bars = 1 mm (A,B), 250 μm (C-E).